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A Practical Guide to Protein and Peptide Purification for Microsequencing

A PRACTICAL GUIDE TO PROTEIN AND PEPTIDE PURIFICATION FOR MICROSEQUENCING

by: Matsudaira, Paul T.
Format: Plastic Comb

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Product Description Why a Second Edition? The Second Edition provides practical answers to the general question, "How can I obtain useful sequence information from my protein or peptide?" rather than the more specific question asked in the first edition, "How can I obtain the N-terminal sequence?" Important new methods include ways of dealing with blocked N termini, computer analysis of protein sequences, and the recent revolution in mass spectrometry. Review Reviews of the First Edition: "Like the evening network television news programs, this book gives a useful summary of the highlights (of protein chemistry) to enable the masses to go forth armed with enough information to survive in the world." --Cell From the Back Cover Why a Second Edition? The second edition provides practical answers to the general question, "How can I obtain useful sequence information from my protein or peptide?" rather than the more specific question asked in the first edition, "How can I obtain the N-terminal sequence?" Important new methods include ways of dealing with blocked N termini, computer analysis of protein sequences, and the recent revolution in mass spectrometry. Highlights of the Second Edition: * Mass spectrophotometric characterization of proteins and peptides * N-terminal sequencing of proteins with blocked N termini * Internal amino acid sequence analysis after protease digestion in-gel and on-blot * Improved microscale peptide purification methods * Computer analysis of protein sequences * New protocols tested and refined through everyday use in authors' laboratories * Updated reference chapter covering all aspects of protein microsequencing|Why a Second Edition? The second edition provides practical answers to the general question, "How can I obtain useful sequence information from my protein or peptide?" rather than the more specific question asked in the first edition, "How can I obtain the N-terminal sequence?" Important new methods include ways of dealing with blocked N termini, computer analysis of protein sequences, and the recent revolution in mass spectrometry. Highlights of the Second Edition: * Mass spectrophotometric characterization of proteins and peptides * N-terminal sequencing of proteins with blocked N termini * Internal amino acid sequence analysis after protease digestion in-gel and on-blot * Improved microscale peptide purification methods * Computer analysis of protein sequences * New protocols tested and refined through everyday use in authors' laboratories * Updated reference chapter covering all aspects of protein microsequencing

Details

Product Code: 9780124802827
ISBN: 0124802826
Publisher: Academic Press
Publication Date: 1993-06-03
Number of Pages: 184 pages
Languages: english
Edition: 2
Dimension: 5.98 x 0.47 x 8.9 inches
Shipping Weight: 0.66 pounds